Kinetic Study of Growth and Penicillin G Acylase Production by Escherichia coli
نویسندگان
چکیده
منابع مشابه
Production of Penicillin Acylase.
The production of penicillin acylase by Escherichia coli Ny.I/3-67 has been increased by phenylacetic acid and phenoxyacetic acid, which themselves strongly inhibit the function of this specific enzyme. Other carbonic acids also increased penicillin acylase production, but to a lesser degree; they also weakly inhibited enzyme function. The production of this enzyme was effectively repressed wit...
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The penicillin G acylase genes from the Proteus rettgeri wild type and from a hyperproducing mutant which is resistant to succinate repression were cloned in Escherichia coli K-12. Expression of both wild-type and mutant P. rettgeri acylase genes in E. coli K-12 was independent of orientation in the cloning vehicle and apparently resulted from recognition in E. coli of the P. rettgeri promoter ...
متن کاملA new crystal form of penicillin acylase from Escherichia coli.
A new crystal form of penicillin acylase (penicillin amidohydrolase, E.C. 3.5.1.11) from Escherichia coli W (ATCC 11105) is reported. The crystals were grown using a combination of hanging-drop and streak-seeding methods. The crystals are in the monoclinic space group P2(1) with cell dimensions a = 51.52, b = 131.95, c = 64.43 A, beta = 106.12 degrees. There is one heterodimer in the asymmetric...
متن کاملImprovement of penicillin G acylase expression in Escherichia coli through UV induced mutations
We used ultraviolet (UV) radiation to induce mutation in three locally isolated strains of Escherichia coli. Different dilutions of bacterial cultures were exposed to UV lamp of 254 nm wavelength for different time intervals at varied distances ranging from 5 to 210 sec and 5 to 100 cm. Viable colonies were screened for mutants with an increased production of penicillin G acylase (PGA) and a re...
متن کاملNew active site oriented glyoxyl-agarose derivatives of Escherichia coli penicillin G acylase
BACKGROUND Immobilized Penicillin G Acylase (PGA) derivatives are biocatalysts that are industrially used for the hydrolysis of Penicillin G by fermentation and for the kinetically controlled synthesis of semi-synthetic beta-lactam antibiotics. One of the most used supports for immobilization is glyoxyl-activated agarose, which binds the protein by reacting through its superficial Lys residues....
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ژورنال
عنوان ژورنال: Pakistan Journal of Biological Sciences
سال: 2000
ISSN: 1028-8880
DOI: 10.3923/pjbs.2000.862.865